1) Preeminently, follow all laboratory security guidelines ensuring the you have actually safety goggles ~ above at all times throughout the lab.
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2) fill the cuvette 2/3 that the method with water.3) pick the appropriate wavelength ~ above the spectrophotometer.
4) place the cuvette in the spectrophotometer and also click the calibrate button.
5) placed 5mL of stock solution right into test tube.6) fill the cuvette 2/3 of the method with the first solution.7) place the cuvette in the spectrophotometer.
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8) Wait a few seconds and also record the absorption on her data table.
9) placed 4mL of stock solution and 1mL the water in a test tube to make the 2nd solution.
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10) to fill the cuvette 2/3 of the means with the 2nd solution.
11) ar the cuvette in the spectrophotometer.
12) Wait a few seconds and also record the absorb on your data table. 13) put 3mL of share solution and also 2mL the water in a test tube to do the 3rd solution. 14) to fill the cuvette 2/3 of the means with the 2nd solution 15) place the cuvette in the spectrophotometer.
16) Wait a few seconds and also record the absorb on her data table. 17) placed 2mL of share solution and 3mL of water in a test pipe to make the fourth solution 18) to fill the cuvette 2/3 of the way with the 4th solution 19) ar the cuvette in the spectrophotometer.
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20) Wait a few seconds and also record the absorb on her data table. 21) placed 1mL of share solution and 4mL the water in a test tube to do the fifth solution 22) to fill the cuvette 2/3 the the way with the fifth solution
23) place the cuvette in the spectrophotometer.24) Wait a few seconds and also record the absorb on her data table. 25) After all data room collected, use them to build a graph identifying the straight relationship between absorbency and concentration. —–
Data and Calculations:Data recorded:Concentration(µM):5.634.3443.2882.1721.0860Absorption:.462.398.294.186.0820Water (mL):012345Stock systems (mL):543210
Linear Regression: y = .088x – .003A = abca = Molar absorptivityb = Path length of the sample cellc = ConcentrationAbsorbency the Gatorade = 0.1150.115 = (130.000)(1)(c)c = 8.85 x 10-7
Conclusion: In this experiment, we prepared solutions and used Beer’s regulation to analyze the concentration the dye in a beverage. The graph would carry out the most accurate way to determine the concentration of an unknown solution and also we understand that absorbance dimensions are many accurate in the selection of A values from 0.2 to 1.0.
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Analysis that Food water in Beverages. (2016, Apr 24). Retrieved from https://medtox.org/analysis-of-food-dyes-in-beverages-essay